Sodimn ferulate preconditioning protect the rabbit lung from ischemia-reperfusion injury

Objective To investigate protective and effects of the sodium ferulate on rabbit lung from ischemia-reperfusion injury by studying serum nitric oxide （NO） and lung tissue nuclear factor-κB 1065 （NF-κB p65）. Methods Reproducing the Sekido modal of the ischemia-reperfusion in rabbit lung, 18 rabbits were divided into 3 groups, the sham group （n = 6） ; ischemic-reperfusion group （n = 6） ： the left lung hilus were clamped to ischemic and then followed by reperfusion as I/R; preconditioning greup（SF, n = 6） ： treated with 100 mg/kg SF before I/R ）. Blood from samples were collected the common carotid at the following time points （before ischemia, ischemia 60 mins, reperfused 30 mins, reperfused 120 mins）, studied for NO. The lung tissues NF-κB p65 was assayed by immunohistochemitry. Results Sodium ferulate preconditioning increased the endogenous NO at the time points 30 rains （28.650±6.185） and 120 mins （33.748 ±6.157）, significant different statistically from I/R group （15.070±8.560, 20.500±4.619,P〈0.01）, and inhibited the NF-κB p65 in SF group （78.852 ± 4.875）, also significandy different from the I/R group （63. 390 ± 1.190）. Conclusion Sodium ferulate can protect the lung from I/R injury by decreasing the destruction of the NO and depressing the NF-κB p65 and thus abates inflammatory lung injury. Key words: Reperfusion injury Sodium Ferulate Preconditioning Nitric oxide Nuclear factor（NF）-κB