An Experimental Study of Bone Mesenchymal Stem Cells Seeds into Small Intestinal Submucosa Induced Toward Bone In Vitro

Objective To investigate the feasibility of bone mesenchymal stem cells(BMSCs) seeded into small intestinal submucosa(SIS) to form the tissue engineered bone through inducing culture.Methods The BMSCs were isolated by percoll gradient centrifugation from rabbit's bone marrow.The BMSCs were induced to become osteogenic cells in the defined medium,and were seeded on SIS as the scaffold material for the formation of cell-SIS composite.The scaffold-cell constructs were cultured in vitro for 1 week and were implanted subcutaneously in the dorsa of nude mice.The implants were examined by scanning electron microscopy(SEM),transmission electron microscopy(TEM),histology and immunohistochemistry.Results BMSCs adhered and proliferated on SIS,secreted a great deal of extracellular matrices with active cellular function.Gross observation showed subcutaneous lump with considerable tenacity.Large amount of osteocyte were seen and the expression of osteocalcin(OCN) were detected by immunohistological staining technique.Conclusion It is demonstrated that BMSCs seed SIS can be induced toward bone by induction media.The SIS is an ideal scaffold for the bone tissue engineering.