한국형 인공피부조직을 이용한 탐색적 피부과민성 대체시험법 개발

Keraskin™-VM, reconstructed human epidermis, was previously investigated for its applicability in development of skin irritation alternative test methods. Considering its expression of various inflammatory mediators, Keraskin™-VM was further evaluated whether it could be useful for development of skin sensitization alternative test method. Keraskin™-VM was incubated 24h in the presence of various skin sensitizing or non-sensitizing test substances followed by collection of culture supernatants. Level of interleukin(IL)-18, a representative pro-inflammatory cytokine, in the supernatants was determined. All the skin sensitizing substances resulted in significantly higher IL-18 levels than the their vehicle control leading to maximum 64-fold, 28-fold, and 85-fold induction of IL-18 expression from 2,4-dintrochlorobenzene, eugenol, and citral, respectively. Among three non-sensitizing test substances (sodium lauryl sulfate, lactic acid, and glycerol), the maximum fold induction of IL-18 was 16 from 0.01% sodium lauryl sulfate. Cell viability was not substantially affected on the magnitude of IL-18 fold induction. Overall, our results suggest the potential usage of Keraskin™-VM for skin sensitization alternative test method.