Monosaccharide composition of polysaccharides in Borojo in tropical rain forest.

The polysaccharide content and the monosaccharide composition in Borojo sample with difference extraction methods were researched.In method 1,the Borojo sample was extracted with ethanol to remove monosaccharides and oligosaccharides and defatted followed by a water extraction and ethanol precipitation.The content of polysaccharides was determined using Phenol-sulfuric acid method and the composition of the polysaccharides in Borojo after acid hydrolysis was analyzed by HPLC-ELSD.In method 2,the Borojo sample was freeze-dried and extracted with ethanol to remove monosaccharides and oligosaccharides.Then the content of polysaccharides was also determined using Phenol-sulfuric acid method and the composition of the polysaccharides in Borojo after acid hydrolysis was analyzed by GC derivatization method.With method 1,the content of the polysaccharide in Borojo sample was determined as 6.2% and the main components of the polysaccharide were found as galactose,arabinose and rhamnose monosaccharide.By using method 2,the content of the polysaccharide was 11.2% and its main components showed to be the galactose,arabinose,mannose and glucose monosaccharide.The content of uronic acid was 0.24% in Borojo sample.It was concluded that Borojo polysaccharide was a kind of complex polysaccharide,composed of most of neutral sugar and small amount of uronic acid.