Response of nutritional growth and photosynthesis to enriching CO2 in Pinellia ternata plantlet of sugar-free tissue culture.

In this paper,adventitious buds of Pinellia ternata were used as material and the response of nutritional growth and photosynthesis to enriching CO2 in P.ternata plantlet of sugar-free tissue culture was discussed.The results showed in the CO2 concentration range of(600～1800)μmol/mol,with the increasing of CO2 concentration,the leaf area,rooting rate,net photosynthetic rate(Pn),Water use efficiency(WUE) and chlorophyll content of P.ternata plantlet of sugar-free tissue culture were raised gradually,but the transpiration rate(Tr) and stomatal conductance(Gs) declined.While the CO2 concentration reached(1500±5)μmol/mol,the Pn reached the highest value of(10.20±0.27)μmol/(m2·s) and lots of fibers grew.So(1500±50)μmol/mol was the optimal CO2 concentration for the photosynthesis of P.ternata plantlet of sugar-free tissue culture.