Effect of dentin adhesives on sealant bond strength.

: This study evaluated the effect of four dentin adhesives on sealant shear bond strength. Five groups of 15 human extracted teeth (75 in total) were prepared to receive the following treatments: group 1, sealant only; group 2, sealant plus gluma primer; group 3, sealant plus gluma primer and sealer, group 4, sealant plus C & B Metabond. Each tooth had a bonding site prepared for each material by grinding a flat plane on the enamel with 600 grit. The enamel site was etched for 30 seconds, washed for 20 seconds and dried. Each material was mixed according to the instructions of the manufacturer. The sealant (Concise) was placed in a nylon cylinder, light-cured, stored in distilled water for 24 hours, thermocycled (100 cycles at 5 to 55 degrees C) and shear bond strength determined with a knife-edge blade in an Instron running at a crosshead speed of 0.5 mm/min. The results in MPa were: group 1, 11.8 +/- 4.5; group 2, 12.3 +/- 5.0; group 3, 10.3 +/- 1.9; group 4, 12.5 +/- 8.6; and group 5, 15.8 +/- 5.2. Statistically (ANOVA one way) there was no significant difference in the groups.