Plasma biomarker discovery in preeclampsia using a novel differential isolation technology for circulating extracellular

CholeratoxinBchain(CTB)andannexinV(AV)whichrespectivelybindsGM1gangliosideandphosphatidylserinewereusedtoisolateextracellularvesiclesfromplasmaofPEpatientsandhealthypregnant women. The proteins in the vesicles were identified usingenzyme-linked immunosorbent assay, antibody array, and massspectrometry.