ISOLATION OF PHOSPHATIDYLINOSITOL FROM SOYBEAN PHOSPHATIDES

A method is described for isolating phosphatidylinositol (PI) from a lipid extract of soybean phosphatides. The method includes the separation of the extract with the aid of a Buchner funnel and alumina into two fractions — choline-containing phospholipids and a PI fraction — and the purification of the latter on a column of DEAE-cellulose. As a by-product, phosphatidylethanolamine with a chromatographic purity of 95–96% is obtained from the PI fraction.