Effect of ascorbic acid on chemiluminescence of cells cultivated in various concentration of oxygen

Objective To investigate the effect of Vitamin C(Vit.C) on the stimulated chemiluminescence of rabbit's pulmonary alveolus macrophage(AM) cultivated in various concentration of oxygen. Method The AMs from rabbits were cultured in a thermostat in which luminescence from cells can be examined,then air with various concentrations of oxygen were continuously led in the device and the AM's stimulated chemiluminescence by PMA(phorbol myristate acetate) was measured with a chemiluminometer. Result In the air with 0.5% O 2,AM's survival rate and luminescence level were only 50% or 45% of the values before the exposure,and were reducing progressively with increasing dose of Vit.C in the culture medium.To increase the concentration of oxygen in the air was advantageous in enhancing cell's vitality and it can partly counteract the decrease of AM's stimulated chemiluminescence level by adding Vit.C into the culture medium. Conclusion Oxygen in the air is important for cultivated cell's luminescence and survival,and it could lead to grave damage to the cultivated AMs when there is high concentration of Vit.C in the culture medium.