Artificial enzymes for Diels‐Alder reactions

Diels-Alder (DA) reaction is a cycloaddition of a conjugated diene and an alkene (dienophile) leading to the formation of a cyclohexene derivative  via  a concerted mechanism.  As DA reaction generally proceeds with a high degree of regio- and stereoselectivity, it is widely used in synthetic organic chemistry.  Considering eco-conscious public and governmental movements, efforts are directed towards the development of industrial processes that meet environmental  concern s. Artificial enzymes, that can be developed to catalyze abiotic reactions, appear as important synthetic tools of synthetic biology field. This review describes the different strategies used for developing protein -based artificial enzymes for DA reaction, including for  in cellulo or in vivo  approaches.