43 The broad use of erythropoietic stimulating agents (ESA) for myelodysplastic syndromes (MDS) in the U.S. is not consistent with guidelines

3. We developed a novel epigenetic profiling-based flow cytometry to measure expressions of nuclear factors at the single cell level. Conclusion: Epigenetic regulatory mechanisms including DNA and histone methylations are important for regulating expression of RUNX1 and PU.1. Identification of epigenetic alterations on these factor encoding genes indicates the importance of epigenetic disregulation in the pathogenesis of MDS.