Characterization of a Triticum aestivum–Dasypyrum villosum T2VS·2DL translocation line expressing a longer spike and more kernels traits

Key message By using 2V-specific EST–PCR markers and sequential GISH/FISH analysis, we identified four homozygous CS–2V translocation lines, including a novel compensating T2VS·2DL translocation line NAU422. This translocation line has longer spikes and produces more grains per spike than its recurrent parent CS and three other translocation lines, which could be a valuable resource in wheat yield improvement.