In vitro evaluation of arbuscular mycorrhizal-like fungi and Trichoderma species against soil borne pathogens

Two arbuscular mycorrhizal like-fungi (Piriformospora indica and Sebacina vermifera), and two species of Trichoderma (Trichoderma viride and Trichoderma harzianum (T-100)) were evaluated against two isolates of Sclerotinia sclerotiorum, two isolates of Fusarium oxysporum f. sp. lentis, and two species of Rhizoctonia (Rhizoctonia solani and Rhizoctonia zeae). Antagonistic fungi against the pathogens in dual culture, volatile metabolite and colonization were evaluated. In dual culture revealed that antagonistic fungi could produce a good zone of inhibition, and T. harzianum (T-100) that was observed maximum growth inhibition on mycelium of two isolates of S. sclerotiorum. The volatile metabolite studies revealed that R. solani was most susceptible to the volatile metabolite produced by T. harzianum (T-100), and colonization revealed that antagonistic fungi were able to overgrow the colony of pathogens and could lyse mycelia .