Effect of Hypertonic Saline Solution on different models of respiratory ephitelium inflammation

Introduction: Years ago HSS began to be used as therapeutic agent in respiratory diseases. Recently experimental studies showed antiinflamatory actions of HSS on respiratory epithelium. Calu-3 is a human respiratory epithelium cell line that was widespread used in physiological and pharmacological studies. Objectives: to evaluate the effect of HSS on the liberation of proinflammatory citokines induced on Calu-3 by 3 different stimulus: addition of poly I:C (viral infection model), of IL1β (chronic inflammatory disease model), and cigarette smoke concentrate. Materials and Methods: Calu-3 (ATCC®Cat HTB55) were cultured in monolayer and stimulated with: a) 10 µg/ml of Poly I:C HMW (InvivoGen), b) IL 1β 50 ng/ml and H2O2 100 µM, and c) CSC 100 µg/ml (Murty Pharmaceutical). All experiments were done in culture media with normal osmolarity (318 mOmol/kg, controls (C)) and in medium with addition of HSS to achieve 410 mOmol/kg (experimental). IL-6 was measured by ELISA (Human ELISA Set, BD Bioscienses) in all suppernatants. In all models, controls of cellular viability were done by colorimetric method (MTS-CellTiter96®). Estatistic analysis: ANOVA and Tukey test. Results: treatment with HSS has no effect on cell viability and reduce IL-6 liberation to medium in poly I:C model (2129.2±269.3 vs C 122.2±71.3 p 0.05 en pg/ml). Conclusions: Our results confirm that antiinflamatory actions of HSS on models related to clinical conditions in therapy with HSS seems to have effect, but also shaws that these effect is not extended to other inflammatory ways