O-3 : Evaluation of changes induced by temperature and storage after dilution in the efficacies of disinfectants against the avian influenza virus

Avian influenza viruses are highly susceptible to all disinfectants because they are enveloped viruses. Disinfectants effective against AIVs have optimum efficacies at temperatures above 20°C. Very few studies on effective disinfectants at low temperatures have been done. The aim of this study was to evaluate the change in the efficacy of 6 disinfectants approved for use against the avian influenza virus (AIV). Disinfectants were investigated at four different temperatures (25°C, 4°C, 0°C, and .10°C) and two contact times (1 min and 5 min) with suspension tests. Further storage time (3, 10, 20, and 30 days) on the efficacy of disinfectants diluted to two working concentration (hard water and organic material condition) also investigated. The results from the suspension test indicated that low temperatures inhibited the virucidal efficacy of citric acid (CA) and citric acid+quaternary ammonium compounds (CA+ QACs) for both 1 min and 5 min, while the remaining disinfectants were effective, regardless of the short contact times and low temperatures. The working concentration with organic material condition may be stored for up to 30 days at room temperatures without loss of effectiveness against AIVs however, in the hard water condition, the efficacy of diluted disinfectants were reduced after 3 days old. For a successful disinfection during winter, the disinfectants which could have short contact times with optimum efficacy and freshly prepared disinfectant performed better in the hard water condition. Acknowledgements: This study was supported by a grant (Z-1541745-2012-13-01) from Animal, Plant & Fisheries Quarantine and Inspection Agency(QIA), Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea in 2012.