Study of ~(99m)Tc-HL91 imaging to monitor hypoxiain mice tumor after hyperbaric of oxygen

Objective To evaluate whether 99m Tc-HL91 hypoxia imagining could monitor the status of tumor hypoxia after hyperbaric oxygen.Methods Twenty bearing H22 tumor KM mice were randomly divided into 4 groups,5 mice/group: The control group: only to carry on 99m Tc-HL91 hypoxia imaging.The 15 minute group,to carry on 99m Tc-HL91 hypoxia imaging at 15 minute after hyperbaric oxygen.The 30 minute group,to perform 99m Tc-HL91 hypoxia imaging at 30 minute after hyperbaric oxygen.The 60 minute group,to perform 99m Tc-HL91 hypoxia imaging at 60 minute after hyperbaric oxygen.ROI were drawn around tumor and contralateral muscles in planar images to calculate the T/NT,then the tumor was excised,weighted and the radioactivity was measured.Differential uptake ratio(DUR) was computed to quantitate the tracer uptake.Results Compared with the control group,the tumor DUR and T/NT of 15 minute group and 30 minute group were significantly different(P0.05),60 minute group suggested no significant difference(P0.05).Conclusion The 99m Tc-HL91 hypoxia imaging might be valuable in evaluating the status of tumor hypoxia after hyperbaric oxygen.