High-performance liquid affinity chromatographic separations: Activation of matrices by 4,6-diphenylthieno[3,4-d]-1,3-dioxol-2-one-5,5-dioxide

Abstract High-performance liquid affinity chromatography requires activated matrices with specific properties. Standard matrices were modified and activated and then their characteristics were compared. A new activating method based on the use of 4,6-diphenylthieno[3,4- d ]-1,3-dioxol-2-one-5,5-dioxide was developed. The coupling of ligands with these new supports was very fast. The hydrolysis/aminolysis ratio was higher than with N-hydroxysuccinimide-activated matrices.