Preparation and characterization of Holothuria nobilis saponins nobilisideA freeze-dried liposome.

Objective:To prepare and characterize nobilisideA freeze-dried liposome. Methods:The nobilisideA liposomes were prepared using a film dispersion method followed by a freeze-dried technique. The freeze-dried liposomes were characterized with regard to the drug content,entrapment efficiency,the particle size,ζ potential,and its stability and hemolytic rate in glucose transfusion. Results:Through optimized technique,the prepared nobilisideA freeze-dried liposome,with sucrose as protective agent,had satisfactory structure and redissolubility. The contents of nobilisideA liposome before and after freeze-drying were 108.3% and 108.6%,the entrapment efficiency were 98.4% and 97.8%,the particle sizes were 89.9 nm and 115.8nm,and the ζ potentials were-19.9mV and-15.9mV,respectively. The liposome content,entrapment efficiency,particle size and hemolytic rate were stable within 8 h during glucose transfusion. Conclusion:NobilisideA freeze-dried liposomes have a narrow size distribution range,a high entrapment efficiency and a stable character,making it a promising candidate for nobilisidesA liposome.