The effect of the polyether antibiotic omomycin on the activity of cyclic nucleotides in the primary culture of newborn rat heart cells

: Content of cyclic nucleotides was studied in primary culture of newborn rat heart cells after administration of the polyether-containing antibiotic omomycin (985-I, 16-diethyl indanomycin). Level of cGMP was increased within 10 min of the cells exposition with 4 micrograms/ml of the antibiotic, while the nucleotide content elevation was more significant in presence of 1-methyl-3-isobutyl xanthine (10(-4) M). When the exposition time was prolonged up to 20 min, content of cGMP returned to control values in absence of isobutyl xanthine and remained at the elevated level in presence of the drug. Content of cAMP was unaltered under these conditions. Omomycin may be used in experiments, where distinct increase of the cellular cGMP content is required.