Comparison of absorption kinetics and utilisation of DL-methionine (DL-Met), Met-Met product (AQUAVI® Met-Met), and protein-bound methionine (PB-Met) by female broiler chickens.

1. Two experiments were conducted to determine the effects of different methionine (Met) sources regarding their absorption kinetics and utilisation in female single-meal-fed broiler chickens.2. A total of 340, one day old female Ross 308 broiler chickens were fed commercial starter and grower diets for 38 d. Birds were then allocated to treatment diets in two experiments as a completely randomised design with four replicates of five chicks per each until 60 d of age. In experiment 1, a 2 × 5 factorial design was used to investigate the effect of two sources (DL-Met and AQUAVI®Met-Met) and five equimolar levels (0.4, 0.8, 1.2, 1.6, and 2 g/kg) in the diet. In experiment 2, different proportions of protein-bound methionine (PB-Met) to DL-Met (0.4:1.6, 0.8:1.2, 1.2:0.8: 1.6:0.4, and 2:0 g/kg) were incorporated into a basal diet deficient in Met. During the experiment, chickens received 90 g of pelleted feed for a time period of 17 ± 2.5 min, once daily.3. The results indicated that chickens fed diets supplemented with DL-Met and Met-Met showed a rapid rise in plasma Met 1 h after feeding, with a sudden drop at 2 h after feeding. In contrast, chickens fed PB-Met substituted diets showed a gradual plasma peak at 1 and 2 h postprandial (P < 0.01). Plasma homocysteine (HCY) content increased to 34.38 and 40.43 μmol/l with DL-Met2.0 and Met-Met2.0 diets, while it decreased to 25.68 μmol/l with PB-Met2.0(P ≤ 0.01). Chickens that received the PB-Met2.0 diet had higher (P ≤ 0.01) protein utilisation (0.54 g/g) and lower excreta nitrogen content (4.04 g/100 g excreta), which demonstrated the benefits of feeding a protein-bound Met source. The efficiency of Met utilisation was 0.69 g/g in chickens fed PB-Met2.0 diet, but only 0.36 and 0.41 g/g in those fed DL-Met2.0 and Met-Met2.0 (P ≤ 0.01).4. The observed utilisation coefficient of DL-Met and Met-Met for single-meal meat-type chickens was lower than expected. The synchronisation of intestinal Met absorption maintained the efficiency of utilisation, which was related to the sources of added Met, with protein-bound Met showing the best utilisation and least excretion.