Production and analyses of transgenic mice with ectopic expression of cellular retinoic acid-binding protein

Transgenic mice with ectopic expression of bovine cellular retinoic acid-binding protein (CRABP) under the control of human metallothionein IIA promoter have been generated. From a total of 10 independent transgenic live borns, six lines have expressed the bovine CRABP messages in a variety of tissues. Among the six lines, three appear to be normal and healthy in the founders as well as their offspring, one has generated transgenic offspring with retarded growth and two have produced only female transgenic mice that are all sterile.