Diagnostics through Ralstonia solanacearum genomics: grasping the broad diversity of a complex plant pathogen. [S4-O4]

designed to identify 17 phylogenetic clusters of diagnostic interest, including species, phylotype or sequevar-specific probes along with control probe . Highlight was given on three ecotypes in the phylotype II: (i) Brown rot trains from sequevars IIB-1 ; (ii) Moko disease-causing srains from sequevars IIB-3 IIB-4, IIA-6 and IIA-24, that cause wilt on banana plantain, and Heliconia; and (iii) new pathogenic variants from sequevar IIB-4NPB that lacks pathogenicity to banana Cavendish but can infect many other plant species, including Cucurbitacae. As a first step, a spotted array glass slide format was chosen for its probe screening capacity, where the 100 best 50mers probes out of more than l 000 were selected on 80 strains for switching to a more convenient microarray format, based on the "Array Tube" technology from Alere Technologies, which allows micro-reactions within a vial. Several advantages to witch to this technology will be presented along with first results. (Resume d'auteur)