Etude du bourgeonnement in vitro de l'écaille du bulbe de Tulipe

Vegetative propagation of tulips in vitro, not performed until now, is obtained using as explants scales of daughter bulbs collected in winter. Twenty-five percent of these explants give rise to neoformed buds within 6 months. These buds evolve into bulbs which are able to develop in the soil. Their ontogeny is studied histologically, from the beginning of the culture to the neoformed bulb formation. A treatment with low temperature and darkness at the beginning of the culture period promotes an earlier bud initiation.