Development and Validation of a Gas Chromatography Method for Quantitative Determination of Lavendar Oil in Lavaflam Preparation

Lavender (Lavandula angustifolia) is a bush in the family Lamiaceae that grows in the Mediterranean region. The material used for phytochemical purposes includes lavender flowers (L. flores) containing essential oil (3%), anthocyans, phytosterols, sugars, minerals, and tanning agents. The qualitative and quantitative compositions of lavender essential oil are variable quantities that depend on genotype, habitat, climate, distribution, and morphological features. Essential oil contains over 300 chemical compounds. The dominant constituents of lavender oil are linalool, linalyl acetate, terpinen-4-ol, lavandulol acetate, and cineol. Lavender essential oil possesses good antioxidant and antimicrobial properties and has a significant positive effect on the gastrointestinal and nervous systems [1]. A monograph on lavender oil was introduced into the State Pharmacopoeia of Ukraine (SPU) [2]. According to the SPU, lavender oil is a transparent, colorless or pale yellow liquid with a characteristic aroma [2]. The SPU regulates the contents of the following main constituents in lavender oil (%) [2]: limonene, 0.2; lavandulol, >0.1; and -terpineol, <2.0. These standards suggest that the principal constituents of lavender oil are linalool and linalyl acetate. Quantitative determination of the listed constituents by gas chromatography used internal normalization. We developed the original combined dosage form Lavaflam containing the constituents flamin (0.05 g) and lavender oil (0.02 g) as the active ingredients for treating gallstones [3]. Pharmacological studies showed that flamin plant substance in combination with lavender oil supplements the comprehensive treatment regime for diseases of the liver and bile duct [4]. The goal of the present work was to develop and validate a procedure for quantitative determination of lavender oil in the combined dosage form Lavaflam.