An endonuclease activity in Bacillus subtilis specific for UV-irradiated DNA

Abstract An enzyme activity specific for UV-DNA ∗ was found in the extract of Bacillus subtilis (Marburg 168). The enzyme preparation obtained from the extract by ammonium sulfate precipitation acts on UV-DNA endonucleolytically and induces single strand breaks. The number of single strand breaks introduced in DNA is proportional to UV dose.