Effect of sinomenine on adjuvant arthritis and its mechanisms

Objective: To discuss the anti-inflammatory mechanism of sinomenine on inflammatory media in joint of adjuvant arthritis rats. Method: Rats were randomly divided into the normal group and the model group, the prednisone group, the small, medium, large dosages of sinomenine group(30, 60, 120 mg·kg~(-1)). Except for the rats in the normal group, animals were modeled to adjuvant arthritis with freund's complete adjuvant. The arthritis index(AI) and the swelling degree of paws were recorded, and the activity of IL-1, TNF and the levels of NO, PGE_(2) in joint fluids of secondary arthritis were determined. Result: Compared with the normal group, the activity of IL-1, TNF and the levels of NO, PGE_(2) in joint fluids of secondary arthritis were increased significantly in the model group(P0.05). Compared with the model group, it was shown to exert a dramatic inhibitory effect on secondary reaction of freund's adjuvant arthritis of rats, and the activity of IL1, TNF and the levels of NO, PGE_2 in joint fluids of secondary arthritis were significantly decreased in the sinomenine group(P0.05). Conclusion: Sinomenine has a remarkable treatment effect on RA. It is via NO to inhibit the activity of cytokines and decrease the level of inflammation mediators, which may be one of its curing RA mechanism.