Virulence and recycling potential of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) from Saharanpur district, western Uttar Pradesh, India

There are complex morphological differences in the species of blow flies associated with the decomposing corpses in different habitats and environments. A thorough diligent examination of each specimen is required for identifying them, as species associated with an exposed corpse differ from those on the buried or burnt body or when the corpse is writhed after death. Molecular data serves as a lucrative tool in identifying insect specimens, suitably when the specimens are damaged and their morphological identification is arduous. Mitochondrial cytochrome oxidase subunit I (COI) DNA of 6 blow fly species were analysed to study its application value as the identification marker. The sequencing of a 350bp COI gene of these six species was done, and the data was eventually analyzed. Phylogenetic tree was constructed based on UPGMA method and which showed similitude in phylogram pattern. Also the sequences obtained for these species were compared with the sequences obtained for them in the other parts of the world for population studies.