Isolation of Gram-positive Bacteria with High G+C from Inside Soil Aggregates

In the present study, we tried to isolate bacterial strains from inside soil aggregates as generally microbial flora inside differs from those outside. In order to effectively isolate microorganisms from inside the soil aggregates, we washed the surface with electrolyzed NaCl solution, which possesses bactericidal activity, then fractured them with an ultrasonic processor. Gram-positive bacteria with high G+C isolated from the inside of soil aggregates were then compared with those isolated from outside on the basis of their 16S rDNA partial sequences. The results showed that the two sets of isolates differ.