SCCRO/DCUN1D-gene family is commonly dysregulated in non-small cell lung cancers

22058 Background: SCCRO1/DCUN1D1 is a novel oncogene mapped to 3q26.3, a locus known to be amplified in a wide range of human cancers. SCCRO1 amplification is associated with an aggressive clinical course. In silico analyses identified four highly conserved SCCRO1 paralogs mapped to chromosomal loci known to be aberrant in malignancies, including non-small cell lung cancer (NSCLC). The aim of this study is to assess the status of the SCCRO/DCUN1D family in NSCLC by gene expression analysis and correlate with histological subtypes of NSCLC, clinical parameters and outcome. Methods: RNA was extracted from snap frozen primary lung cancer from 56 patients undergoing definitive resection following institutional guidelines and after obtaining informed consent (n=27 adenocarcinomas (ADC); n=30 squamous cell carinomas (SCC)). Specific primer pairs were designed. Gene expression analysis was performed using RT-PCR. GEnorm identified B-actin as the most stable housekeeping gene which was used as control. Change in ...