Dobutamine alters carnitine metabolism in the neonatal piglet heart.

The use of inotropic agents to support the neonatal heart after sepsis or hypoxia increases cardiac energy demand. Carnitine plays a vital role in energy, fuel metabolism. To test the hypothesis that inotropic agents affect carnitine metabolism, hearts from sow-fed piglets were isolated and perfused with an oxygenated buffer containing glucose and palmitate. Increasing dosages of dobutamine (DOB 2.5–15 µg/Kg body wt per min, 0.007–0.044 µmol/kg per min) or saline vehicle (SAL) were administered. Heart rate (HR), left ventricular systolic (LVSP) and end diastolic pressures (LVEDP) were measured. Left ventricular developed pressure (LVDP = LVSP - LVEDP) and pressure-rate product (LVDP × HR) were calculated. Coronary effluent was collected to measure flow and metabolites. Heart tissue samples were collected for metabolite analysis. Results: DOB increased HR, LVEDP and the pressure-rate product [LVDP × HR]. Mean lactate production increased in DOB, but not in SAL control hearts, and was correlated with heart ...