Archaeocyte is divided into the cultural method of functionally ripe egg mother cell

The subject of the invention is to provide a kind of methods that archaeocyte being divided into function GV phase egg mother cells by vitro culture.The present invention relates to a kind of methods that archaeocyte being divided into function GV phase egg mother cells by vitro culture comprising：(a) by under conditions of the influence of elimination estrogen or the factor with function similar with estrogen, cultivating the sertoli cell of archaeocyte and neighbouring archaeocyte come the step of generating secondary follicle；(b) the step of partly cutting the combination between granular cell layer and theca cell layer, wherein egg mother cell, granular cell layer and theca cell layer constitute the secondary follicle generated；(c) by cultivating egg mother cell, granular cell layer and the theca cell layer of composition secondary follicle in the culture medium containing high-molecular compound, egg mother cell being made to be divided into function GV phase egg mother cells the step of.