Coherent Anti-stokes Raman Spectroscopy with Dual-Wavelength Oscillation Electronically Tuned laser

Coherent anti-stokes Raman scattering (CARS) spectroscopy or imaging is used for investigating the chemical or biological dynamics in living samples without staining or fluorescent proteins. Different two wavelengths and synchronization of pulses generated from individual lasers by using optical delay line are required to obtain the CARS signal. So the excitation light source and the signal include the jitter noises occurred when the wavelengths are tuned and the pulse timing is synchronized. The stability of laser intensity is most important and required factor to obtain the CARS spectrum with high signal to noise ratio and achieve quantitative measurements of living samples.