Abstract 3011: Evaluation of a high throughput platform for chromatin immunoprecipitation

Expression of eukaryotic genes during development requires complex spatial-temporal regulation. This complex regulation is often achieved through the coordinated interaction of transcription regulatory elements in the promoters of the target genes. The identification and mapping of regulatory elements in genome scale is crucial to understand how gene expression is regulated Chromatin immunoprecipitation is a standard method for assessing the occupancy of DNA binding proteins in vivo in their native chromatin context using antibodies. However, standard chromatin immunoprecipitation procedure is time consuming, labor intensive and not suited for analyzing many samples simultaneously. Recently, we have developed a simple chromatin immunoprecipitation protocol, which utilizes high capacity protein A/G coated magnetic beads and 96 well plate. This protocol requires fewer steps, little hands-on time and is compatible with multi-channel pipetting and liquid handling robot. This protocol allows genome wide mapping of transcription regulatory network in a high throughput manner. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3011. doi:10.1158/1538-7445.AM2011-3011