Biofunctionalization of Si nanowires using a solution based technique
2012
Here we present a solution based functionalization technique for streptavidin (SA) protein conjugation to silicon
nanowires (Si NWs). Si NWs, with a diameter of 110 nm to 130 nm and a length of 5 μm to 10 μm, were functionalized
with 3-aminopropyltriethoxysilane (APTES) followed by biotin for the selective attachment of SA. High-resolution
transmission electron microscopy (HRTEM) and atomic force microscopy (AFM) showed that the Si NWs were
conformally coated with 20 nm to 30 nm thick APTES, biotin, and SA layers upon functionalization. Successful
attachment of each bio/organic layer was confirmed by X-ray photoelectron spectroscopy (XPS) and fluorescence
microscopy. Fluorescence microscopy also demonstrated that there was an undesirable non-specific binding of the SA
protein as well as a control protein, bovine serum albumin (BSA), to the APTES-coated Si NWs. However, inhibition of
BSA binding and enhancement of SA binding were achieved following the biotinylation step. The biofunctionalized Si
NWs show potential as label-free biosensing platforms for the specific and selective detection of biomolecules.
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