2-(2-Phenylethyl)chromones from Endophytic Fungal Strain Botryosphaeria rhodina A13 from Aquilaria sinensis

Abstract Objective To study the characteristic 2-(2-phenylethyl)chromone components of endophytic fungal strain of Aquilaria sinensis by solid culture. Methods The compounds were isolated by various chromatographic methods such as silica gel, reverse-phase silica gel, Sephadex-LH20 column chromatography as well as crystallization. Results Seven 2-(2-phenylethyl)chromone analogues were isolated from the solid culture of Botryosphaeria rhodina A13. Their structures were established by spectral data as well as physicochemical properties, and identified as 6-hydroxy-7-methoxy-2-(2-phenylethyl)chromone ( 1 ), 6,7-dimethoxy-2-(2-phenylethyl) chromone ( 2 ), (5 S ,6 R ,7 S ,8 R )-2-(2-phenylethyl)-5,6,7,8-tetrahydrchromone ( 3 ), 6-hydroxy-2-(2-phenylethyl)chromone ( 4 ), 4′-hydroxy-2-(2-phenylethyl)chromone ( 5 ), 6-methoxy-2-phenethyl-4 H -chromen-4-one ( 6 ), and 6-methoxy-2-(4′-methoxy-phenethyl)-4 H -chromen-4-one ( 7 ). Conclusion All of the compounds are isolated for the first time from the genus Botryosphaeria. This research opens up a new vista to produce the characteristic components of agarwood by endophytic fungi.