Application of microsatellite makers for motherless parentage testing in Alopex lagopus.

This study was initiated to assess the feasibility of motherless parentage testing by microsatellites in order to give help for family accuracy determination in bluefox(Alopex lagopus).15 genotypes of the most polymorphic microsatellites loci were detected in 6 families(6 female parents,61 offsprings)of bluefox.Gene frequency,expected heterozygosity(H),polymotphism information content(PIC),Exclusion Probability(EP),Cumulative Chance of Exclusion(CCE),Paternity Index(PI),Relative Chance of Paternity(RCP)were calculated by tested 67 bluefoxes in 6 families.The results showed that the Cumulative Chance of Exclusionof 15 microrsatellites loci was 0.999 996,the Paternity Index of Motherless Parentage Testing were between 850.521 3 and 966 160,and RCP were between 99.882 6% and 99.999 9%.The results of this study revealed that the microsatellite markers could be used in the Motherless Parentage Testing of blue fox with high assignment rate and accuracy.