Preparation and specificity analysis of sildenafil polyclonal antibody.

Objective To prepare high sensitivity and specificity polyclonal antibody against sildenafil(SD). Methods Sildenafil hapten(SDH) and Sildenafil antigen(SDH-BSA) were prepared by artificial methods. Then, through immunized rabbits the SD polyclonal antibody was prepared. Results After immunity, the polyclonal antibody against SD was generated. The titer of the antibody was 3.8×105. The linear detection of the polyclonal antibody was ranged from 0.02 ng/m L to 3.8 ng/m L. The polyclonal antibody had a high cross reaction rate to vardenafil, but had a low response rate to hongdenafil and calais. Conclusion SD polyclonal antibody had a high specificity and sensitivity, and it could be applied to the development of SD rapid detection kit.