Loop-mediated isothermal amplification as alternative to PCR for the diagnosis of extra-pulmonary tuberculosis.
2015
BACKGROUND: The main challenge in combatting
extra-pulmonary tuberculosis (EPTB) is the lack of a
rapid, reliable and inexpensive diagnostic test for the
detection of Mycobacterium tuberculosis.
OBJECTIVE : To evaluate the diagnostic potential of an
L-serine dehydratase gene (sdaA) loop-mediated isothermal
amplification (LAMP) assay for the detection of
M. tuberculosis in clinical specimens from presumptive
EPTB patients.
METHODS : An in-house sdaA LAMP assay was used to
analyse clinical specimens (n¼315) for the diagnosis of
EPTB compared with culture and the composite
reference standard (CRS) comprising culture and polymerase
chain reaction (PCR) using insertion sequence
(IS) 6110 and mpb64 as target genes.
RESULT S : The sdaA LAMP assay showed the highest
sensitivity (93.3%) in comparison to culture; the
sensitivity of IS6110 PCR, mpb64 and sdaA PCR assay
was respectively 80%, 86.7% and 90%. In comparison
to CRS, the LAMP assay had a sensitivity of 92.5% and
a specificity of 99.2%, with a high positive (121.11) and
a low negative likelihood ratio (0.08).
CONCLUS ION: Due to its speed, simplicity, sensitivity
and specificity, the sdaA LAMP assay is a potential
diagnostic test for the diagnosis of EPTB, particularly in
resource-limited settings.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
15
Citations
NaN
KQI