Structural transformations and aggregation stability of lecithin liposomes in cryoprotective media

To study the structural transformations and aggregation stability in liposomes, a new approach is proposed whose efficiency is demonstrated during the analysis of light scattering by lecithin liposome sols doped with low-molecular cryoprotectors . This approach is used to determine absolute dimensions, relative values of weight concentration, masses of vesicules and their surface densities as well as their polydispersity characteristics. The anisotropic scattering data suggest that cryoprotectors eliminate small scale defects which are partially restored at low temperatures. Low-molecular cryoprotectors are found to produce some favourable changes in dimensions and distribution of liposomes and to increase their aggregation and structural stability.