Análise genotípica de sorovares de Bacillus thuringiensis através do RAPD-PCR

In this study, genomic DNA fi ngerprints of 40 Bacillus thuringiensis serovars representing different serotypes were obtained by Randomic Amplifi cation of Polymorphic DNA (RAPDPCR) using three different randomic primers. The electrophoretic analysis of RAPD band profi les showed the presence of polymorphism among the serovars and serotypes. Between the primers used in this study, primer 2 was the most discriminatory and, therefore, was used to build the dendrogram. The similarity analysis of the electrophoretic profi les showed low level of similarity (16%) among the different B. thuringiensis serovars. The serovars were divided into two main groups, and these indicated a high level of genetic divergence both intra and inter-group. Results suggested that genomic fi ngerprints obtained by RAPD can be used for the genotypic characterization and identifi cation of B. thuringiensis serovars as a complement to fl agellar serology.