EVALUATION OF ANTIBIOTIC RESISTANCE MODEL OF ESCHERICHIA COLI IN URINE CULTURE SAMPLES AT KIAN HOSPITAL LAB IN TEHRAN, 2011-2012

Background and purpose: Urinary tract infection (UTI) is one of the most common infections and the second cause of infection in human body. In developing countries it involves at least 250 million people annually. The main cause of this infection is gram negative bacteria such as E coli. UTI is seen in all ages and both sexes but its incidences is more in female. The base of treatment for urinary tract infection is administrating an appropriate antibiotic. Today, antibiotic resistance is considered as an important challenge in treating infections. High and unnecessary use of antibiotics especially in urinary infections resulted in resistance to some antibiotics. Therefore, this study was designed to assess the antibiotic susceptibility patterns of Ecoli strains isolated from urine samples of urinary infected patients. Material and Methods: In this study urine samples were collected applying Midstream clean catch method and cultured in blood agar and EMB medium using standard loop then incubated for 24hr in 37 0 . Results: During 12 months, 1147 samples were studied of which, 208 samples were reported positive. The rate of positive cultures was found higher in women and their mean age was less than that of men. The main antibiotics resistant to Escherichia coli were nalidixic acid, cotrimoxazole, and cefazolin and the most sensitivity was found with imipenem, nitrofurantoin, and piperacillin. Conclusion: According to this study, urinary infection of Ecoli is more common amongst women of younger ages. In this study Ecoli showed lower resistance to imipenem, nitrofurantoin and piperacillin. Therefore, these antibiotics are the most effective in controlling Ecoli.