Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography–mass spectrometry: Application to polytraumatized patients

Abstract Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F 2 -isoprostanes (F 2 -IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo . Since “interferences peaks” at m / z 573 co-elute with d 4 -15-F 2t -IsoP preferentially used, we propose a new GC–NICI-MS approach to quantify urinary F 2 -IsoPs by using 4( RS )-F 4t -neuroprostane as the internal standard. This method was applied to quantify urinary F 2 -IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase ( p 2 -IsoPs in polytraumatized patients compared with healthy volunteers (4.73 ± 2.75 ng/mg vs. 0.811 ± 0.359 ng/mg creatinine).