REGENERATION OF PLANTLETS FROM GRAPE (Vitis vinifera L.) THROUGH DIFFERENT EXPLANTS

Chowdhury MMH, Ashrafuzzaman M, Begum SN, Islam MM, Dhar P (2012) Regeneration of plantlets from grape (Vitis vinifera L.) through different explants. Int. J. Sustain. Crop Prod. 7(2), 12-18. The experiment was conducted to investigate the effect of different plant growth regulators on growth and plantlet regeneration of grape ‘Zakkao’cultivar by tissue culture technique. Among the different concentration of BAP at 2.5 mg/l supplemented to LS medium was the best response for callus induction and moderate shoot initiation and BAP 2.0 mg/l + 0.5 mg/l NAA for plantlet regeneration, shoot development from shoot tip, node and leaf explants. Maximum shoot height and shoot fresh weight was obtained from the BAP 2.0 mg/l+0.5 mg/l of NAA. For rooting, half strength MS medium with 3.0 mg/l IBA showed best performance in the respect of root length and root fresh weight from node, shoot tip and leaf explants derived callus. An efficient observation found that for in vitro grapevine regeneration, only half strength MS medium without hormonal combination was effective for root and shoot induction from node and shoot tip explants.