Synthetic compound non-human animal model unresponsive to an immunopotentiating effect

Gene function encoding TLR7 recognizing the synthetic compound having an immunopotentiating effect of such imidazoquinoline compound is deficient on its chromosome, there is provided a synthetic compound non-human animal model unresponsive. All or part of the gene fragment of the gene site containing an intracellular domain and a transmembrane region of TLR7 gene obtained from a mouse gene library, to build a targeting vector by replacing the plasmid with the poly A signal and a marker gene , and introduced into embryonic stem cells after the targeting vector was linearized, the target embryonic stem cells lacking TLR7 gene function, microinjected to produce a chimeric mouse blastocysts of mice, wild-type mice and the chimeric mice mated the door to prepare a heterozygous mice to produce a TLR7 knockout mice by intercrossing such heterozygous mice.