Isolation of nuclear rapidly sedimenting ribonucleic acids by agarose gel filtration

Abstract Gel filtration of rat liver nuclear RNA's on Sepharose 4B permits routine separation of the rapidly sedimenting (30–60S) ribopolynucleotides from ribosomal RNA's. The method could be easily adapted to either analytical characterization or preparative isolation of preribosomal and polycistronic RNA's.