Recovery and enhancement of unsaturated fatty acids in soft-shelled turtle fish oil using supercritical carbon dioxide and associated catalase release activity

Abstract This work studied concentration enhancement of omega-3 fatty acids extracted from soft-shelled turtle fish ( Pelodiscus sinensis ) oil bags by using supercritical carbon dioxide (SC-CO 2 ). Crude oil was obtained by using top-down pressurized carbon dioxide extraction at 60 bar and 328 K. Crude oil was then ethylated and concentrated using a counter-current SC-CO 2 extractive distillation at 200 bar and 353 K. 81 wt% of unsaturated fatty acids (i.e. C 20–24 ) of this ethylated oil was successfully obtained in the raffinate of the distillation. The reflux flow rate, the carbon dioxide-to-oil ratio and the extraction temperature were found to be important in affecting this concentration of the C 20–24 . The column-height versus concentration of C 20–24 was examined using the Riccati equation. Furthermore, the catalase activity of rat macrophage RAW 264.7 treated with the SC-CO 2 raffinate was investigated.