cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province.

The cDNA of coat protein gene and its 3' non coding region of tobacco mosaic virus and coat protein gene of cucumber mosaic virus isolated from the tobacco in Yunnan province was synthesized by RT PCR. The amplified cDNA fragments were then cloned and sequenced. The fragment of TMV YN comprises 684bp (EMBL accession number: AJ239099), including 480bp of CP gene encoding 158 amino acids and a 204bp of 3' non coding region. Comparison either the nucleotide sequence of CP gene or that of 3' non coding region demonstrated that the TMV YN is identical to the TMV U1 strain and TMV common strain in Korea, with 100% homology. The CP gene of CMV YNa consists of 657nt encoding 218 amino acid (EMBL accession number: AJ239098). The nucleotide sequence of CMV YNa has 96%, 93% and 92% nucleotide identities with CMV RB, CMV 117F and CMV Y, respectively.