Stimuli-responsive microgels with fluorescent and SERS activities for water and temperature sensing.

Abstract Design and application of stimulus-responsive microgels is still in its infancy but is an exhilarating topic in controllable sensing device. Here, we have fabricated a dual-responsive platform capable of both sensitive on-spot fluorescence analysis and reliable surface-enhanced Raman scattering (SERS) quantification of water and temperature by in-situ encapsulating 4,4′-dimercaptoazobenzene (DMAB), meso-formyl-1,3,5,7-tetramethyl pyrromethene fluoroborate (FPF) probe and Ag nanoparticles (AgNPs) into polyvinyl alcohol (PVA) microgels. The smart microgels exhibit ultra-sensitive (detection limit 10−4% v/v) and reversible response towards water due to the liner relationship between network volume and SERS performance of the microgels. Furthermore, the presence of water triggers the conversion of FPF to aldehyde hydrate, facilitating visual assay of trace water in matrix samples through the enhanced fluorescence signals. Interestingly, the SERS signals can be precisely tuned by the thermo-sensitive microgels substrate, thus achieving the temperature monitoring from 32 to 50 °C. The microgels-based sensor has fast-response (2 min), excellent stability, and enables accurate and reliable response of water in organic solvent and pharmaceutical products. As a smart and flexible sensor, the hybrid microgels will facilitate the field of POC analysis, as well as molecular recognition in the future.