Screening Psychrophilic Fungi of Cellulose Degradation and Characteristic of Enzyme Production

A fungus（WR-C1） decomposed cellulose was isolated from a hypothermal litter layer using Congo red medium as the preliminary screening culture medium and then using a filter as the secondary screening medium at low temperature. The experiment showed that the weight loss rate of filter paper on the 15 th days could reach 30.69%. A morphologic and ITS gene sequence analysis suggested that CF-C1 was Cladosporium. We mainly studied the effects of culture time, inoculation amount, initial p H and different sources of carbon, nitrogen and inorganic salt on the cellulase production of strain WR-C1. Under optimum cultural condition, the highest value of WR-C1 enzyme production and filter paper enzyme were 3.27 U · m L-1 and 0.51 U · m L-1.