Synthesis and binding properties of myo-inositol 4,5,6-tris(phosphate) an analogue of myo-inositol 1,4,5-tris(phosphate). Correlation with the ionization state of the molecules

Abstract Myo -inositol 4,5,6-tris(phosphate) is synthetized. The binding activity for this analogue with the CNS Ins(1,4,5)P 3 receptors is explained by taking into account steric modification and, the ionization state of the entire molecule, determined by potentiometric analyses, and the ionization state of each phosphate group determined by 31 P-NMR studies.